El cultivo de protoplastos en cítricos y su potencial para el mejoramiento genético. Article (PDF Available) · July with 1, Reads. Export this citation. Results 80 – 90 Download Citation on ResearchGate | On Jan 1, , R.R. Rodríguez and others published Aislamiento y cultivo de protoplastos en maracuyá. Universidad de León – Grado en Biotecnología – Tercero Biotecnología – Biotecnología vegetal – Tema 9. Cultivo de protoplastos e hibridación somática.
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The authors would like to thank the International Potato Center for financial support and to Martha Williams de Castro who revised the English dee of this article.
This can be done via morphological, biochemical, cytological and molecular markers Pinto et al. Identification of somatic hybrids is a requisite for the effective exploitation of the protoplasts fusion in potato improvement. After seven days, the culture medium was diluted 1: RAPD markers for confirmation of somatic hybrids in the dihaploid breeding of potato Solanum tuberosum L. Chromosome observations indicated that all hybrids were aneuploids. A slot-blot hybridization method for screening somatic hybrids.
Somatic potato hybrids have been produced by fusion of diploid wild species with tetraploid S. It has been demonstrated that protoplast-derived plants show a higher degree of chromosomal number variation than those derived from tissue or organ culture. Identification of somatic hybrids of dihaploid S. Isolation of plant Protoplaatos from fresh tissue. Analysis of chromatin, nuclear DNA and organelle composition in somatic hybrids between Solanum tuberosum and Solanum sanctae-rosae.
Isolamento e cultivo de protoplastos de porta-enxertos de citros
Genetic Resourses Conservation and Characterization Division. The wild species S. Earn while you study. Plant Cell Rep 3: Tissue culture procedures to produce somatic hybrids and the application of molecular biological tools for their analysis are well established. Earn hundreds of dollars each month by selling your written material to your fellow students. Root tips were stained by lacto-propionic orcein. For this analysis, DNA extraction was performed according to Doyle and Doyle with some modifications.
Regenerants from the fusion experiments were propagated in vitro using the Murashige and Skoog medium. In this study, confirmation of fusion-derived regenerants by the use of RAPD markers represented an effective system for the detection of somatic hybrids from S. Interspecific somatic hybrids between wild potato Solanum acaule Bitt.
Tema 9. Cultivo de protoplastos e hibridación somática
Regeneration of fertile plants from Helianthus nuttalli and Helianthus giganteus L. Regeneration in Gewebekulturen einiger Dihaploider Solanum tuberosum Z. Production and analysis of intraspecific somatic hybrids of potato Solanum tuberosum L. These barriers in classical breeding can be overcome using biotechnological methods such as somatic hybridization prptoplastos protoplast fusion Carputo et al.
Cultivo y fusion de protoplastos 
Acknowledgments The authors would like to thank the International Potato Center for financial support and to Martha Williams de Castro who revised the English version of this article. RAPD and chromosomal analysis of the hybrids are also presented. A rapid calli proliferation was observed and most of them showed purple-colored cells Fig.
The morphology of fusion-derived regenerants was intermediate between the donor parents. Thanks to the study guides written by fellow students specifically for your courses, you will never miss a trick when it comes to your exams.
Nineteen somatic hybrid plants were obtained from fusion experiments using PEG in an effort to combine elite protoplasto from both parents. Molecular characterization of inter- and intra-specific somatic hybrids of potato using randomly amplified polymorphic DNA RAPD markers. After that, protoplasts settled down.
Tissue culture response in various wild and cultivated Solanum germplasm accessions for exploitation in potato breeding. Shoots also showed purple-colored meristematic zones.
After that, colonies were dispersed into plates with solid MS13K regeneration medium Cutlivo, Segundo curso Primer curso. The ploidy of the genotype used in plant regeneration has been shown to profoundly affect the type of variation observed protoplqstos morphological characters and chromosome numbers among regenerant plants. This study shows that somatic hybrid potato plants can be obtained by the fusion method presented.
Protoplasts fusion from S. Have you written lots of study guides or notes?
Protoplasts were precipitated by centrifugation at rpm during 10 minutes.